Figure 1. Typical FACS Profiles for EasySep™ Mouse Monocyte Isolation Kit

Starting with mouse bone marrow cells, the monocyte content (Lineage- (CD3, CD45R, CD117, CD49b, Siglec F) CD11b+Ly6G- Ly6Chi/lo) of the isolated fraction is 89.5 ± 4.8% (mean ± SD), using the purple EasySep™ Magnet. In the above example, monocyte purities in the start and final isolated fractions are 7.1% and 92.3%, respectively.

Figure 2. Data for Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 (Catalog #60001AD) and anti-mouse CD45 APC. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19861) and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 (Catalog #60001AD). Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram). (D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19861) and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 (Catalog #60001AD). Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Figure 3. Cell Isolation Protocol Lengths

Typical time taken (in minutes) to isolate cells using select EasySep™ kits.