Figure 1. Differentiation of mouse compact bone-derived mesenchymal stem cells (CB-MSCs) with the MesenCult™ Osteogenic Stimulatory Kit (Mouse)

Compact bone-derived MSCs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 2. Compact bone-derived MSCs display robust bone matrix deposition following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse) as demonstrated by Alizarin Red-staining
Ca2+ deposits are detected with Alizarin Red.
For both the qPCR analysis and Alizarin-Red staining assay, the mouse compact bone-derived MSCs were cultured in vitro for three passages, purified with EasySep™ Mesenchymal Stem/Progenitor Cell Enrichment Kit (Mouse, Catalog #19771), and then cultured for another passage, prior to differentiation with the MesenCult™ Osteogenic Stimulatory Kit (Mouse).

Figure 3.Differentiation of mouse embryonic fibroblasts (MEFs) with MesenCult™ Osteogenic Stiumulatory Kit (Mouse)
MEFs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 4. MEFs treated with MesenCult™ Osteogenic Stimulatory Kit (Mouse) displayed substantially increased alkaline phosphatase activity and bone matrix deposition*
Alkaline phosphatase activity is shown by Naphthol AS-MX Phosphate/Fast Red Violet LB Salt staining (red areas) while bone matrix deposition is shown by von Kossa staining (black areas).
*Versus a homemade osteogenic medium (mouse) from Lab A.
For the qPCR analysis and staining assays, MEFs were cultured in vitro for 20 days using MesenCult™ Osteogenic Stimulatory Kit (Mouse), homemade, or MesenCult™ Proliferation Kit (Mouse, Catalog #05511) as the negative control.