Figure 1. Human Mesenchymal Stem and Progenitor cells (MSCs) Derived and Culture-Expanded Using the MesenCult™ Proliferation Kit

(A) Human bone marrow mesenchymal stem and progenitor cells (BM MSCs) were derived and culture-expanded for 8 passages in complete MesenCult™ Proliferation Medium (n = 6) and two commercially available media (Commercial Medium 1 and Commercial Medium 2; n = 3). (B) The average doubling time in days over 8 passages of human BM MSCs culture-expanded in complete MesenCult™ Proliferation Medium, and Commercial Medium 1 and 2. Vertical lines indicate standard error of the mean.

Figure 2. Human MSCs Culture-Expanded in Complete MesenCult™ Proliferation Medium Retain Strong Multi-Lineage Differentiation Potential

(A) Human BM-derived MSCs cultured in complete MesenCult™ Proliferation Medium differentiate into (B) adipocytes (Oil Red O staining), (C) chondrocytes (Alcian Blue staining) and (D) osteoblasts (alkaline phosphatase and von Kossa staining).

Figure 3. Flow Cytometric Analysis of Culture-Expanded Human MSCs in Complete MesenCult™ Proliferation Medium

Human BM-derived MSCs from passage 5 were stained for mesenchymal cell surface markers CD73, CD90 and CD105, and the hematopoietic markers CD34 and CD45 (shown in grey shaded areas). BM-derived MSCs are positive for MSC markers and negative for hematopoietic markers. High expression of the endothelial marker, CD146, was also observed in BM-derived MSCs (shown in grey shaded areas). Isotype control is shown by red line.