Figure 1. Comparison of Cell Expansion for Mouse Neurospheres Cultured with Complete NeuroCult™ Proliferation Medium (Mouse & Rat) or a Traditional Formulation

Cells microdissected from the cortices of E14 mice were cultured in Complete NeuroCult™ Proliferation Medium (Mouse & Rat) or a traditional formulation containing 20 ng/mL rh EGF. At Day 71, cells cultured in Complete NeuroCult™ Proliferation Medium (Mouse & Rat) were at Passage 13 while cells cultured in a traditional medium formulation were at Passage 10. Complete NeuroCult™ Proliferation Medium (Mouse & Rat) consists of NeuroCult™ NSC Basal Medium (Mouse & Rat), NeuroCult™ NSC Proliferation Supplement (Mouse & Rat) and 20 ng/mL rh EGF.

Figure 2. Cell Expansion for Rat Neurospheres Cultured with Complete NeuroCult™ Proliferation Medium (Mouse & Rat)

Cells microdissected from the cortices of E18 rat were cultured in 3 different lots of NeuroCult™ Proliferation Medium (Mouse & Rat). In each sample, cells continued to generate neurospheres beyond passage 5, resulting in an increase in total cell number. At passages 1 and 5, cells dissociated from the neurospheres were able to differentiate into neurons, oligodendrocytes and astrocytes [data not shown].